Sun Fei

Nickel Titanium Alloy News 2021-06-24 09:21 68
Research direction:

our main research interest is the structure and function of biological macromolecular complexes (such as membrane protein and supramolecular protein complexes). Our research goal is to integrate a variety of structural biology research methods (mainly freeze electron microscopy and Protein Crystallography) and develop new structural research methods to study the high-resolution structure of biological systems on multiple spatial scales (from nano to meso), multiple time scales (from static to dynamic) and multiple levels (in vitro and in vivo). We take the development of frontier scientific research as the core task, and pay attention to the cultivation of high-quality talents. In the next five years, our research group will focus on three aspects: in situ structural biology technology and application, ultrafast freezing electron microscopy technology and application; Three dimensional structural biology and its application

After more than 30 years of development, especially in recent years, cryoelectron microscopy, especially single particle technology, has become one of the main means of structural biology. The in situ structure analysis of protein machine is the next breakthrough in structural biology, especially the development of high-resolution three-dimensional reconstruction technology of frozen electron tomography. However, due to the difficulties in sample preparation, low integrity and signal-to-noise ratio of raw data, and complex image analysis, the resolution of most protein in situ structure analysis is still above 20 Angstrom. Our research interests are focused on the development of a complete technology platform for in-situ structural analysis, the improvement and optimization of the sample preparation technology by freezing ion thinning, the data acquisition method of tilt sequence, the new algorithm of three-dimensional reconstruction of electronic tomography, and the high-throughput and high-precision three-dimensional positioning, alignment and clustering analysis algorithm of protein quality machine. Using this technology platform, we will successively analyze the in situ high-resolution structures of a series of important protein complexes

The electron irradiation damage of biological samples is an important factor limiting the resolution improvement of cryoelectron microscopy, and the ultrafast cryoelectron microscopy technology provides a new opportunity to solve this problem. The successful combination of ultrafast electron microscopy and freeze electron microscopy will enable us to achieve low radiation damage and high-resolution imaging of protein machines, and study the dynamic structural changes of protein machines at multiple time scales. There is no systematic research on this field in the world. Our research interest is to focus on the development of biological ultrafast frozen electron microscopy technology. On the one hand, we can explore the deep mechanism of pulsed electron irradiation damage of biological samples, and try to break through the bottleneck of signal-to-noise ratio caused by electron irradiation damage; On the other hand, the time resolution of the important protein machine such as super light harvesting complex was imaged by frozen electron microscope to analyze the dynamic process of its time resolution

Three dimensional structural biology and its application the three-dimensional ultrastructure of tissues and organs is of great significance for human beings to understand nature and treat diseases. The breakthrough in volume electron microscopy and the progress in sample preparation technology enable the three-dimensional visualization of samples to achieve high detail, thus promoting the super-resolution of the cell system to achieve a satisfactory degree of reproduction. With the development of ultra-thin microtome with automatic collection and focused ion beam scanning electron microscope, continuous sectioning technology becomes more and more automatic. The development of these technologies has expanded the boundary of biomedicine. Our research interest is to cooperate with the team of bioimaging center to focus on the establishment and development of serial section electron microscopy imaging technology, especially the development of image registration and mosaic, automatic image recognition and separation based on artificial intelligence technology and other processing technologies, and use this technology to cooperate with relevant units to carry out three-dimensional ultrastructure research of multiple tissues and organs

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